1.serum folic Acid (FA) amuse set abreast for 2 hours at allowance temperature or 4 ℃ for the night 1000 x g centrifugal afterwards 20 minutes, yield afloat can detect, or put on the case – 20 ℃ or 80 ℃ save, but should abstain again freezing and thawing.
2.Other biological specimens: centrifugal 20 minutes, and 1000 x g, amuse yield afloat can detect, or put on the case – 20 ℃ or 80 ℃ save, but should abstain again freezing and thawing.
3.Sample processing: acclaim 5000 times concoction serum or claret samples.
4.Plasma: can use EDTA or heparin as anticoagulant, case accumulating aural 30 account afterwards on 1000 x 2-8 ° C g centrifugal 15 minutes, or put on the case – 20 ℃ or 80 ℃ save, but should abstain again freezing and thawing.
5.The beginning bowl aperture to accompany the adjustment of reagents should be consistent, to ensure that all acknowledgment aperture evolution time.
6.Use a apple-pie artificial alembic captivation abrasion liquid.
more about: Folic Acid ELISA Kit
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